Correlation of CMA3 Staining with Sperm Quality and Protamine Deficiency
نویسندگان
چکیده
منابع مشابه
Chromomycin A3 Staining Indicates Sperm Protamine Deficiency
Chromomycin A3 binds to DNA and Protamine -deficient spermatozoa look bright yellow,whereas normal spermatozoa appear dull yellow.
متن کاملP-6: Flow Cytometry: Assessment of Protamine Deficiency by CMA3 Staining and Apoptotic Bodies
Background: Chromomycin A3 (CMA3) staining, with the slide method or fluorescence microscopy, is widely used for indirect assessment of protamine deficiency in a semen sample. Flow cytometry is the most suitable tool which to improve assessment accuracy, both in terms of statistical analysis and to prevent observer variation. This study provides a simple procedure to account for merocyanine 540...
متن کاملCredibility of Chromomycin A3 Staining in Prediction of Fertility
Background Chromomycin A3 (CMA3) staining has been used to assess protamine deficiency. The aim of this study was to determine credibility of CMA3 along with semen parameters for assessment of fertility potential. MaterialsAndMethods Semen analysis and CMA3 staining were carried out on 234 fertile and 178 subfertile individuals. Semen analysis was assessed according to WHO criteria. Protamine d...
متن کاملEffect of sperm DNA damage and sperm protamine deficiency on fertilization and embryo development post-ICSI.
The aim of this study was to evaluate the effect of sperm DNA damage and protamine deficiency on fertilization and embryo development post-intracytoplasmic sperm injection (ICSI), and also to assess the effect of protamine deficiency on DNA damage. Semen samples were collected from 28 patients participating in the ICSI programme. Following sperm preparation and ICSI, the remaining processed sem...
متن کاملP-13: Comparison of Sperm Quality, Oxidative Stress, DNA Fragmentation, Protamine Deficiency, and DNA Methylation in Varicocele and Fertile Individuals
s:1993:"Background: There are many approaches that gene expression is controlled in eukaryotes. DNA methylation is one of several epigenetic mechanisms that cells use to control gene expression and lock genes in the "off" position. In addition, sperm DNA damage can correlate with DNA methylation defect. There is evidence that sperm of infertile men contain more DNA damage than fertile men and t...
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ژورنال
عنوان ژورنال: Laboratory Medicine
سال: 2012
ISSN: 0007-5027,1943-7730
DOI: 10.1309/lmb42f9qxykfljng